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CJC-1295 Reconstitution Calculator

Research Use

Calculate precise U-100 syringe units, concentration (mg/mL), and doses per vial for CJC-1295 research.

Clinical Precision Tool

CJC-1295 Dosage Calculator

U-100 Calibrated
Calculated Syringe Draw
10.0Units
➜ 0.100 mL volume
0 Units100 Units Max
Concentration
1.00 mg/mL
Tick Marks
10.0 ticks
OE
Written by OnePeptideShip Editorial Team
Reviewed by Dr. Aris Thorne, MD, Board Certified Endocrinology Advisor
Updated September 2026

Clinical Pharmacology & Mechanism of Action

CJC-1295 is a synthetic analogue of growth hormone-releasing hormone (GHRH). It is a tetrasubstituted 29-amino acid peptide that mimics the natural action of endogenous GHRH.

Its primary mechanism of action is to bind to GHRH receptors on the anterior pituitary gland, signaling it to release large pulses of natural Growth Hormone (GH) into the bloodstream .

Importantly, CJC-1295 does not replace your body's natural GH. Instead, it acts as a "secretagogue," encouraging your pituitary to produce more of its own.

This maintains the natural pulsatile rhythm of GH release, preventing the pituitary shutdown associated with synthetic HGH injections.

The DAC vs. No DAC Distinction

There is immense confusion regarding the naming convention of this peptide:

  • CJC-1295 No DAC (Modified GRF 1-29): This version lacks the Drug Affinity Complex. It has a very short half-life of roughly 30 minutes, producing a massive but brief spike in GH.
  • CJC-1295 with DAC: The addition of the Drug Affinity Complex allows the peptide to bind to serum albumin in the blood. This extends the half-life from 30 minutes to up to 8 days, causing a slow, constant bleed of GH rather than a sharp pulse.

Pharmacokinetics & Dosing Synergies

CJC-1295 is almost never researched in isolation. It only acts on one half of the pituitary's GH release mechanism.

To maximize GH output, researchers must simultaneously suppress Somatostatin (the hormone that inhibits GH release).

Therefore, CJC-1295 is universally stacked with a Growth Hormone Releasing Peptide (GHRP), most commonly Ipamorelin. When GHRH (CJC-1295) and GHRP (Ipamorelin) are administered together, the GH release is not just additive, it is exponentially synergistic.

Standard Research Dosing Protocols

Because "No DAC" is the standard for anti-aging and recovery research (to maintain natural GH pulses without GH bleed side effects), the following applies to CJC-1295 No DAC:

  • Dosage: 100mcg per injection.
  • Frequency: 1 to 3 times daily (usually morning, post-workout, and before bed).
  • Administration Condition: Must be administered on an empty stomach (at least 2 hours after a meal containing carbohydrates or fats), as insulin blunts GH release.

Reconstitution Math & Syringe Guide

CJC-1295 is highly potent and dosed in very small microgram (mcg) quantities.

Step-by-Step Reconstitution (2mg Vial)

  1. Swab the rubber stoppers of your 2mg CJC-1295 vial and your Bacteriostatic Water.
  2. Using a sterile mixing syringe, draw exactly 2mL of Bacteriostatic Water.
  3. Inject the water into the 2mg vial slowly, aiming at the glass wall.
  4. Swirl gently to dissolve.

The Math for a Standard Dose (100mcg):

  • 2mg total in the vial = 2,000mcg.
  • You added 2mL of water.
  • Therefore, the concentration is 1,000mcg per 1mL.
  • You need a 100mcg dose. Divide the dose by the concentration: 100 ÷ 1,000 = 0.1mL.
  • 0.1mL = 10 units on a U-100 syringe.

Common Mistakes & Troubleshooting

  • Eating Food Before Injection: Administering CJC-1295 while blood glucose or insulin levels are elevated renders the peptide practically useless. A strict fasting window is required.
  • Buying "With DAC" by Mistake: Constant GH bleed from DAC can lead to side effects like carpal tunnel syndrome, water retention, and severe lethargy. Always double-check which version you are purchasing.
  • Shaking the Vial: GHRH peptides are fragile. Vigorous shaking will destroy the peptide chains.

Medical & Research Disclaimer

The information provided on this page, including all calculations, is for educational and laboratory research purposes only. It does not constitute medical advice. Peptides mentioned here are not approved for human consumption outside of FDA-approved clinical trials unless specified otherwise. Always consult a licensed healthcare provider before undertaking any new therapy.

Frequently Asked Questions

No DAC (also called Modified GRF 1-29 or Mod GRF) has a half-life of roughly 30 minutes, creating a sharp, natural-like pulse of GH. It is dosed daily. With DAC adds a Drug Affinity Complex that allows it to bind to albumin, extending the half-life to approximately 8 days, creating a continuous, steady release of GH. It is dosed 1-2 times per week. No DAC is preferred for most anti-aging and recovery research because it preserves the natural pulsatile rhythm of GH.

Using the standard 2mg vial with 2mL of BAC water (concentration: 1,000mcg/mL): 100mcg = 0.1mL = 10 units on a U-100 syringe. This is a clean, easy-to-draw volume.

Yes, and it is highly recommended. Both peptides should be administered at exactly the same time for maximum GH synergy. Pull your CJC-1295 dose (e.g., 10 units) into the syringe first, then pull your Ipamorelin dose (e.g., 20 units for 200mcg) into the same syringe. You get a single injection with an exponentially enhanced GH pulse.

Carbohydrates and fats spike insulin levels. Insulin and Growth Hormone operate on a counter-regulatory seesaw: when insulin is high, GH is suppressed. Injecting CJC-1295 with high insulin means the pituitary receives the GHRH signal but the downstream GH pulse is blunted or eliminated by insulin antagonism. You must be in a fasted state (2+ hours post-meal) for the injection to be effective.

100mcg per injection, administered 1-3 times daily. The most critical time is right before bed, as this coincides with the body's largest natural GH pulse during slow-wave sleep. Pre-workout and upon waking are additional dosing windows. Each injection must be done on an empty stomach.

No, this is its key safety advantage over synthetic HGH. CJC-1295 works within the body's existing feedback loop - when GH levels rise too high, the pituitary naturally produces somatostatin to inhibit further release. This self-limiting mechanism makes CJC-1295 much safer than injectable HGH, which overrides this feedback completely.

Most researchers run CJC-1295 for 12 to 24 weeks for meaningful anti-aging and body composition results, as GH secretagogue effects accumulate slowly. Cycles can be run continuously (especially with No DAC due to its short half-life and natural pulse pattern) or in 16-week-on, 4-week-off cycles.

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