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TB-500 (Thymosin Beta-4) Research Profile

Research Use

TB-500 is a synthetic version of the active region of Thymosin Beta-4, a naturally occurring peptide present in high concentrations in blood platelets and wound fluid. It primarily upregulates actin to promote systemic cell migration and tissue repair.

TB-500 (Thymosin Beta-4) Quick Technical Specifications

Category
Healing & Recovery
Elimination Half-Life
approx. 24 hours (systemic clearance)
Storage Protocol
Refrigerated (2°C-8°C)
Status
Research Use
OE
Written by OnePeptideShip Editorial Team
Reviewed by Dr. Aris Thorne, MD, Board Certified Endocrinology Advisor
Updated September 2026

Mechanism of Action & Biochemical Context

TB-500 is a synthetic version of the active region of Thymosin Beta-4, a naturally occurring peptide present in high concentrations in blood platelets and wound fluid. It primarily upregulates actin to promote systemic cell migration and tissue repair.

Storage, Reconstitution & Stability Guidelines

Prior to reconstitution, lyophilized TB-500 (Thymosin Beta-4) powder should be stored away from light between 2°C and 8°C for short-term access, or -20°C for long-term preservation. Once reconstituted with Bacteriostatic Water (0.9% benzyl alcohol preservative), maintain strict refrigeration between 2°C and 8°C. Do not freeze liquid solution.

Cited Scientific References

  1. Goldstein AL, et al. Thymosin beta4: actin-sequestering protein and tissue repair factor. Expert Opin Biol Ther. 2012.
  2. Sosne G, Kleinman HK. Key aspects of thymosin beta4 biology related to wound healing. Expert Opin Biol Ther. 2015.

Calculate TB-500 (Thymosin Beta-4) Syringe Units

Input your vial mg and BAC water mL to calculate exact U-100 draw units.

Open TB-500 (Thymosin Beta-4) Calculator →

Frequently Asked Questions

With a 10mg vial, adding 2mL of BAC water creates a high concentration of 5mg/mL. For a 2.5mg dose, you draw 0.5mL (50 units on a U-100 syringe). Alternatively, using 4mL creates a more manageable 2.5mg/mL solution, making a 2.5mg dose exactly 1.0mL (100 units). Choose the dilution that gives you the most accurate draw volume.

It depends on your water volume. With 2mL in a 5mg vial (2.5mg/mL): 2.5mg = 100 units (a full syringe). With 2mL in a 10mg vial (5mg/mL): 2.5mg = 50 units. Always use the calculator above with your actual vial size and water volume.

No. Unlike BPC-157, TB-500 is highly systemic due to its very low molecular weight. The peptide travels freely through the bloodstream and will reach damaged tissues throughout the entire body. A standard subcutaneous injection in the abdomen is fully effective - there is no clinical need to inject near the injury site.

A standard research protocol uses a loading phase for the first 4-6 weeks: 4-10mg total per week, split into two injections (e.g., 2.5mg on Monday and 2.5mg on Thursday). After the loading phase, the maintenance phase involves 2-5mg per month, usually administered as one 2mg injection every two weeks, as the half-life keeps levels elevated.

The Wolverine Stack refers to running BPC-157 and TB-500 simultaneously. They work synergistically because they target different healing mechanisms: BPC-157 drives angiogenesis and localized gut/tissue repair via VEGF, while TB-500 promotes systemic cell migration and muscle fiber repair via actin upregulation. They can even be drawn into the same syringe.

TB-500 promotes angiogenesis (new blood vessel formation) as part of its healing mechanism. Since tumors also need blood vessels to grow, there is a theoretical concern that TB-500 could accelerate tumor growth in subjects with pre-existing, active cancer. It does NOT cause cancer itself. Research subjects with a history of cancer should not use TB-500.

Reconstituted TB-500 with Bacteriostatic Water is stable in the refrigerator (2-8°C) for approximately 28-30 days. The dry, unmixed (lyophilized) powder can be stored in the freezer for up to 2 years. Do not freeze reconstituted peptide solutions.